P53 offers a tumor suppressor gene that is expressed in many tissue types and can also be involved in the regulation of cell growth, apoptosis, and replication of cells. It can be bound to MDM2, the human papilloma virus’s E6 protein, and V40T antigens. It can also sense damage to the DNA and can help with repair in some cases. Mutations involving this antibody can be found in many malignant tumors, including melanoma, bladder, colon, lung, breast, and ovaries.
The P53 antibody has a clone called SPM514. The immunogen is the recombinant human protein of the same name. It also has an isotype called IgG20/K, an undetermined epitope and a molecular weight of 53kDa.
This antibody can be used in Immunohistochemistry applications. To prepare your specimen, you should choose a Formalin-fixed or paraffin-embedded tissue sample. Deparaffinized slides can be completed using graded alcohols and xylene, along with a xylene alternative.
When using the concentrated version of the formula, you will need to dilute it using a ratio of 1:400, though this is an estimate and your methods/protocols may call for different dilutions. You can also find a pre-diluted formula that can reduce the steps needed for testing and work well for dilution ratios of 1:400.
To retrieve the antigen, you must boil the tissue section for 10 minutes using a 10mM Citrate buffer with a pH of 6.0. Afterward, you must wait 20 minutes for it to cool back to room temperature. Once at room temperature, allow the concoction to incubate for at least 30 minutes.
The positive control is the colon carcinoma with cellular localization occurring in the nucleus.
The P53 antibody can be used to test a variety of tumors throughout the human body. Visit Spring Bioscience now to learn more.