The P53 antibody is a tumor-suppressor gene that is expressed in many tissue types and can help with apoptosis, replication, and regulation of cell growth. It binds to the SV4oT and MDM2 antigens, as well as the human papilloma virus E6 protein. It can also sense DNA damage and may even be able to help with repairs. Mutation of these antibodies can lead to malignant tumors in the ovary, breast, bladder, lung, colon, and melanoma.
The P53 antibody is designed to be used for research and has a clone called SPM 514. The immunogen is the recombinant human protein of the same name. The isotype is the IgG20/K, and it does not have a determined epitope. Likewise, the molecular weight is 53kDa, and it has been tested on humans.
It is designed to be used with Immunohistochemistry (IHC) applications only. To prepare the specimen, you should choose Formalin-fixed or paraffin-embedded tissues. Deparaffinized slides are necessary, which can be cleaned with xylene and any alternative to xylene, as well as graded alcohols.
You’ll find pre-diluted forms, as well as concentrate forms of this antibody. When choosing the concentrated version, you should dilute it using a ratio of one to 400, depending on your methods and protocols. Likewise, the pre-diluted format may not be suitable for everyone, so it’s best to determine your methods first.
You should boil the tissue section in a 10mM citrate buffer with a pH of 6.0 for 10 minutes, allowing it to cool to room temperature for 20 minutes to retrieve the antigen. It must also be incubated for 30 minutes at room temperature before you can see the visualization.
The P53 antibody is an excellent source to learn more about tumors and how to repair or suppress them. Visit Spring Bioscience now for more information about this and other antibodies.